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Alexa Fluor® 594 anti-human VEGFR1 Antibody

Pricing & Availability
Clone
A16083C (See other available formats)
Regulatory Status
RUO
Other Names
Fms-related tyrosine kinase 1, oncogene FLT, FLT, FLT-1, VEGFR1, VEGFR-1, VEGFR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16083C_A594_VEGFR1_Antibody_100118_updated
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human VEGFR1 (clone A16083C) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A16083C_A594_VEGFR1_Antibody_100118_updated
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human VEGFR1 (clone A16083C) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability Save
394903 25 µg $159
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394904 100 µg $347
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Description

VEGF-R1, also known as Fms-related tyrosine kinase 1 (FLT-1), is type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases.  Human VEGF-R1 is mainly expressed by endothelial cells, which plays central role in vasculogenesis, angiogenesis and lymphaniogenesis. VEGFR-1 is the only VEGFR present on the surface of monocytes, and it mediates a chemotactic response to VEGF-A and tissue factor induction. It also reported on placental trophoblast cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. VEGFR1  is regulated by PEDF, which effects its intracellular proteolysis rate. sVEGFR1 regulates the availability of VEGFA and is produced in the placenta under hypoxic conditions. The ligands of VEGF-R1 include VEGF-A, VEGF-B, and PIGF splice isoforms.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VEGFR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Product Citations
  1. Lawrence M, et al. 2022. Sci Adv. 8:eabj8618. PubMed
RRID
AB_2783307 (BioLegend Cat. No. 394903)
AB_2783307 (BioLegend Cat. No. 394904)

Antigen Details

Structure
Cytokine receptor
Distribution

Endothelial cells, placental trophoblast cells, peripheral blood monocytes and macrophages

Function
Angiognesis and vasculogenesis
Cell Type
Endothelial cells, Macrophages, Monocytes
Antigen References
  1. Kendall RL, et al. 1996. Biochem Biophys Res Commun. 226:324.
  2. Cai J, et al. 2006. J Biol Chem. 281:3604.
  3. Ambati BK, et al. 2006. Nature. 443:993.
  4. Heydarian M, et al. 2009. Placenta. 30:250.
  5. Thomas CP, et al. 2009. J Clin Endocrinol Metab. 94:2524.
  6. Wu, et al. 2010. J Cell Mol Med. 14:528.
  7. Cai J, et al. 2011. PLoS One. 6:e18076.
Gene ID
2321 View all products for this Gene ID
UniProt
View information about VEGFR1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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