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Alexa Fluor® 594 anti-MAP2 Antibody

Pricing & Availability
Clone
SMI 52 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 2, MAP-2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-52_A594_MAP2_Antibody_120817
IHC staining of Alexa Fluor® 594 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.) (Cat. No. 928502), the tissue was permeabilized with 0.25% Triton X-100, and subsequently stained with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The images were captured with A) 4X objective, B) 10X objective and C) 40X objective.
  • SMI-52_A594_MAP2_Antibody_120817
    IHC staining of Alexa Fluor® 594 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.) (Cat. No. 928502), the tissue was permeabilized with 0.25% Triton X-100, and subsequently stained with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The images were captured with A) 4X objective, B) 10X objective and C) 40X objective.
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801802 25 µg $129
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801803 100 µg $323
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Description

Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against Microtubule-Associated Protein (MAP2)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The following applications have been Reported in the literature, not verified in house for relevant formats: ICC5,6, ELISA7, and WB1,5.

SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c.

Application References

(PubMed link indicates BioLegend citation)
  1. Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
  2. Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
  3. Wang X, et al. 2015.PLOS ONE. 10: 0145441. (IHC) PubMed
  4. Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
  5. Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
  6. Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
  7. Gensel JC, et al. 2009.J. Neursci 29: 3956-3968. (ELISA) PubMed

 

Product Citations
  1. Albanese A, et al. 2020. Sci Rep. 10:21487. PubMed
  2. Schlüter A, et al. 2020. Mol Ther Methods Clin Dev. 0.903472222. PubMed
  3. Ku T, et al. 2020. Nat Methods. 17:609. PubMed
RRID
AB_2721382 (BioLegend Cat. No. 801802)
AB_2721382 (BioLegend Cat. No. 801803)

Antigen Details

Structure
MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau).
Distribution

Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol.
Tissue distribution: Predominantly in the nervous system

Function
MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.
Interaction
MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
Ligand/Receptor
MAP2 isoforms are expressed in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhou Y, et al. 2015. Neuro Oncol. 17(12):1578.
2. Loeser H, et al. 2015. Am J Nephron. 41(3):191.

Gene ID
17756 View all products for this Gene ID 25595 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org
Go To Top Version: 1    Revision Date: 12/11/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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