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Alexa Fluor® 594 anti-Phosphotyrosine Antibody

Pricing & Availability
Clone
PY20 (See other available formats)
Regulatory Status
RUO
Other Names
p-Tyr
Isotype
Mouse IgG2b, κ
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Product Citations
publications
PY20_A594_Phosphotyrosine_Antibody_021418
NIH3T3 cells were non-treated (A-C) or treated with 1mM pervanadate for 30 min (D-E) and then were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with 1: 5000 diluted (0.1 µg/ml, A and D), 1: 2000 diluted (0.25 µg/ml, B and E) and 1: 500 diluted (1 µg/ml, C and F) Alexa Fluor® 594 anti-Phosphotyrosine Antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • PY20_A594_Phosphotyrosine_Antibody_021418
    NIH3T3 cells were non-treated (A-C) or treated with 1mM pervanadate for 30 min (D-E) and then were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with 1: 5000 diluted (0.1 µg/ml, A and D), 1: 2000 diluted (0.25 µg/ml, B and E) and 1: 500 diluted (1 µg/ml, C and F) Alexa Fluor® 594 anti-Phosphotyrosine Antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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309313 25 µg $124
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309314 100 µg $265
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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated phosphotyrosine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 0.25 μg/ml (1:500 - 1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
  2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
  3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
  4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
  5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
  6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
RRID
AB_2728248 (BioLegend Cat. No. 309313)
AB_2728248 (BioLegend Cat. No. 309314)

Antigen Details

Distribution

Phospho-Specific

Function
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
5800 View all products for this Gene ID
UniProt
View information about Phosphotyrosine on UniProt.org
Go To Top Version: 1    Revision Date: 02/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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