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Alexa Fluor® 594 anti-Rat Blood-Brain Barrier Antibody

Pricing & Availability
Clone
SMI 71 (See other available formats)
Regulatory Status
RUO
Other Names
Endothelial barrier antigen, EBA
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
A_SMI-71_A594_BBB_Antibody_1_032618
IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_SMI-71_A594_BBB_Antibody_1_032618
    IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_SMI-71_A594_BBB_Antibody_2_032618
    IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_SMI-71_A594_Rat_Blood_Brain_Barrier_IHCP_040318
    IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrier antibody (clone SMI 71) and Alexa Fluor® 488 anti-GFAP antibody (clone SMI 25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibodies at 5 µg/mL overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
Compare all formats See Alexa Fluor® 594 spectral data
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836807 25 µg $129
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836808 100 µg $323
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Description

SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Ghabriel MN, et al. 2002. Reproduction 123:389-97. (IHC)
  2. Beaumont M, et. al. 2009. J. Cereb. Blood Flow Metab. 29:1714. (IHC) PubMed
  3. Sternberger NH, Sternberger LA. 1987. Proc. Natl. Acad. Sci. USA 84(22):8169. (IHC) PubMed
RRID
AB_2728552 (BioLegend Cat. No. 836807)
AB_2728552 (BioLegend Cat. No. 836808)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
NA
UniProt
View information about Blood-Brain Barrier on UniProt.org
Go To Top Version: 1    Revision Date: 03/28/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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