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Alexa Fluor® 647 anti-Asialo-GM1 Antibody

Pricing & Availability
Clone
Poly21460 (See other available formats)
Regulatory Status
RUO
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly21460_Asialo-GM1 _AF647_FC_030714.jpg
C57BL/6 mouse splenocytes were stained with NK1.1 PE and anti-Asialo-GM1 (clone Poly21460) Alexa Fluor® 647.
  • Poly21460_Asialo-GM1 _AF647_FC_030714.jpg
    C57BL/6 mouse splenocytes were stained with NK1.1 PE and anti-Asialo-GM1 (clone Poly21460) Alexa Fluor® 647.
Compare all formats See Alexa Fluor® 647 spectral data
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146003 25 µg $171
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146004 100 µg $422
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Description

GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12. 


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Naiki M, et al. 1974. J. Immunol. 113:84.
  2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
  3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
  4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
RRID
AB_2563195 (BioLegend Cat. No. 146003)
AB_2563195 (BioLegend Cat. No. 146004)

Antigen Details

Structure
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
NK cells, Basophils, T cells, Neurons
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID
NA
UniProt
View information about Asialo-GM1 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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