Alexa Fluor 647 anti-Cyclin B1 Antibody anti-Cyclin B1

Pricing & Availability

Clone: V152 (See other available formats)
Regulatory Status: RUO
Other Names: CCNB1
Isotype: Mouse IgG1
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Product Citations: publications

V152_A647_CyclinB1_Antibody_1_101016 — Jurkat cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and Cyclin B1 (clone V152) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).

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647905

25 tests

$147

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647906

100 tests

$323

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Description

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hamster Cyclin B1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications include: immunohistochemistry² of formalin-fixed paraffin-embedded sections and immunofluorescence¹ of methanol fixed cells grown on glass coverslips.
RRID: AB_2632637 (BioLegend Cat. No. 647905)
AB_2632637 (BioLegend Cat. No. 647906)

Antigen Details

Structure: Belongs to the cyclin family. Cyclin AB subfamily.
Distribution: Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
Interaction: CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Morgan DO, et al.1995. Nature 374:131
Gene ID: 891 View all products for this Gene ID
UniProt: View information about Cyclin B1 on UniProt.org

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Other Formats

View All Cyclin B1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Cyclin B1	V152	WB,ICC,IHC
PE anti-Cyclin B1	V152	ICFC
Alexa Fluor® 647 anti-Cyclin B1	V152	ICFC
Alexa Fluor® 488 anti-Cyclin B1	V152	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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