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Alexa Fluor® 647 anti-DBN1 Antibody

Pricing & Availability
Clone
W21139F (See other available formats)
Regulatory Status
RUO
Other Names
DBN-1, DBN 1, D0S117E; Developmentally-regulated brain protein; Drebrin 1; Drebrin; Drebrin E; Drebrin E2; DKFZp434D064
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W21139F_AF647_DBN1_Antibody_1_091924
HeLa and HeLa shRNA knockdown cells were fixed with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature. Cells were intracellularly stained with Alexa Fluor® 647 anti-DBN1 (clone W21139F). Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µm
  • A. W21139F_AF647_DBN1_Antibody_1_091924
    HeLa and HeLa shRNA knockdown cells were fixed with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature. Cells were intracellularly stained with Alexa Fluor® 647 anti-DBN1 (clone W21139F). Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µm
  • B. W21139F_AF647_DBN1_Antibody_2_091924.
    HEK293T cells were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with 1X Intracellular Staining Permeabilization Wash buffer (Cat. No. 421002), then intracellularly stained with Alexa Fluor® 647 anti-DBN1 (clone W21139F) (filled histogram) or Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 400626) (open histogram).
See Alexa Fluor® 647 spectral data
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633253 25 µg $180
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633254 100 µg $455
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Description

Drebrin (DBN1) is an actin-binding protein that was identified as a developmentally regulated protein expressed in the brain. Drebrin interacts with microtubules and is localized to focal adhesions, adherens junctions, gap junctions, immunological synapses, and neuronal synapses to modulate cellular processes such as cell migration, cell process formation, cancer metastasis, and spermatogenesis. There are two isoforms of mammals as a result of alternative splicing: Drebrin A (adult) and Drebrin E (embryonic). The expression of Drebrin A is highly specific to neurons and regulates the reorganization of actin filaments and thus contributes to memory, whereas Drebrin E is expressed in various tissues. Studies have shown that a decrease in the amount of Drebrin protein in the brain is thought to be a factor contributing to the pathogenesis of dementia in Alzheimer's disease (AD), Down syndrome (DS), and bipolar disorder (BD).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human DBN1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended.  For flow cytometric staining, the suggested range of this reagent is 0.125 - 0.25 μg/mL µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation.

For intracellular flow cytometry (ICFC), the recommended dosage is 0.25 μg per test using Fixation Buffer (Cat. No. 420801) followed by True- Phos™ Perm Buffer (Cat No. 425401), or Intracellular Staining Permeabilization Wash Buffer (Cat No. 421002), or using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

This product is not recommended for use in immunohistochemistry (IHC).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for ICC.

Antigen Details

Structure
DBN1 protein is a 649 amino acid protein with a predicted molecular weight of 71 kD.
Distribution

Expressed in the brain, the heart, lymphocytes, placenta, lung, skeletal muscle, kidney, pancreas, skin fibroblasts, gingival fibroblasts, and bone-derived cells

Function
Cytoplasmic actin-binding protein, regulate dendritic spine morphogenesis and organization
Interaction
RUFY3, CXCR4, ZMYND8
Cell Type
Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kojima N, et al. 1993. Brain Res Mol Brain Res. 19:101-14.
  2. Ishikawa R, et al.1994. J Biol Chem.269:29928-33.
  3. Butkevich E, et al.2004. Curr Biol. 14:650-8.
  4. Shirao T, et al. 2017. J Neurochem. 141:819-834.
  5. Peitsch WK, et al. 1999. Eur J Cell Biol. 78:767-78.
  6. Li MW, et al. 2011. Spermatogenesis. 1:123-136.
  7. Geraldo S, et al. 2008. Nat Cell Biol. 10:1181-9.
  8. Grintsevich EE, et al. 2017. Adv Exp Med Biol. 1006:61-82.
  9. Stiber J.A, et al. 2016. Arterioscler Thromb Vasc Biol.36:984-93.
  10. Shim K.S, et al. 2002. Neurosci Lett. 324:209-12.
  11. Kim H.W, et al. 2010. Neurobiol Dis. 37:596-603.
Gene ID
1627 View all products for this Gene ID
UniProt
View information about DBN1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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