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Alexa Fluor® 647 anti-FOXA1 Antibody

Pricing & Availability
Clone
2F83 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_2F83_A647_FOXA1_Antibody_2_040519.png
MCF-7 cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with anti-FOXA1 (clone 2F83) Alexa Fluor® 647 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • 1_2F83_A647_FOXA1_Antibody_2_040519.png
    MCF-7 cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with anti-FOXA1 (clone 2F83) Alexa Fluor® 647 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • 2_2F83_A647_FOXA1_Antibody_1_040519.png
    HEL cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with anti-FOXA1 (clone 2F83) Alexa Fluor® 647 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
612003 25 µg $176
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612004 100 µg $440
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Description

FOXA1 is a transcriptional activator that is required for tissue-specific differentiation during embryonic development. It also functions as a coactivator with nuclear hormone receptors, including ESR1, and has been shown to play a major role in driving estrogen receptor positive breast cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human FOXA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles.

Additional Product Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Besenard V, et al. 2004. Gene Expr Patterns. 5:193-208. (IHC-P)
RRID
AB_2801117 (BioLegend Cat. No. 612003)
AB_2801117 (BioLegend Cat. No. 612004)

Antigen Details

Structure
FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD.
Distribution

Nucleus/Early endoderm derived cells

Function
Transcriptional activator
Interaction
FOXA2, NKX2-1, Histones H3 and H4
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
3169 View all products for this Gene ID
UniProt
View information about FOXA1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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