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Alexa Fluor® 647 anti-human Aiolos Antibody

Pricing & Availability
Clone
16D9C97 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF3, AIO, ZNFN/A3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16D9C97_A647_Aiolos_Antibody_1_033116
Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 488, treated with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Aiolos (clone 16D9C97) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 16D9C97_A647_Aiolos_Antibody_1_033116
    Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 488, treated with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Aiolos (clone 16D9C97) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 16D9C97_A647_Aiolos_Antibody_2_033116
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371105 25 tests $159
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371106 100 tests $393
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Description

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
RRID
AB_2572171 (BioLegend Cat. No. 371105)
AB_2572171 (BioLegend Cat. No. 371106)

Antigen Details

Structure
58kD, zinc finger protein.
Distribution

T and B lymphocytes.

Function
Hematopoetic-specific transcriptional activator and chromatin remodeler.
Interaction
Forms heterodimers with Ikaros family members IKZF4 and IKZF5.
Cell Type
T cells, B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319.
2. Ma S, et al. 2008. Blood. 111:1396.
3. Billot K, et al. 2010. Leuk. Res. 34:289.
4. Morgan B, et al. 2004. EMBO J. 16:2004.
5. Wang JH, et al. 1998. Immunity. 9:543-53.
6. Hosokawa Y, et al. 1999. Genomics. 61:326.

Gene ID
22806 View all products for this Gene ID
UniProt
View information about Aiolos on UniProt.org
Go To Top Version: 1    Revision Date: 03/31/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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