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Alexa Fluor® 647 anti-human CD195 (CCR5) Antibody

Pricing & Availability
Clone
HEK/1/85a (See other available formats)
Regulatory Status
RUO
Other Names
CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype
Rat IgG2a, κ
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Product Citations
publications
HEK185a_Alx647_020708
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Alexa Fluor® 647 (filled histogram), or rat IgG2a, κ Alexa Fluor® 647 (open histogram).
  • HEK185a_Alx647_020708
    Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Alexa Fluor® 647 (filled histogram), or rat IgG2a, κ Alexa Fluor® 647 (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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313711 25 tests $147
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313712 100 tests $300
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Description

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with human CCR5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1

Clone HEK/1/85a and clone HEK/1/85a/7a are the same.

Application References

(PubMed link indicates BioLegend citation)
  1. Mueller A, et al. 2002. Blood 99:785.
  2. Al-Odi E, et al. 2012. PLoS One. 7:e42217. PubMed
  3. Osman A, et al. 2014. J Immunol. 192:5083. PubMed
  4. Liu S, et al. 2014. Virus Res. 192:92. PubMed
Product Citations
  1. Park A, et al. 2016. Mol Ther Methods Clin Dev. 3:16057. PubMed
  2. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  3. Piryani SO, et al. 2019. Stem Cell Reports. 13:76. PubMed
  4. Achour L, et al. 2008. Blood. 101182. PubMed
  5. Mandl M, et al. 2014. PLoS One. 9:112140. PubMed
  6. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  7. Tadagaki K, et al. 2012. Blood. 119:4908. PubMed
  8. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
RRID
AB_528759 (BioLegend Cat. No. 313711)
AB_528759 (BioLegend Cat. No. 313712)

Antigen Details

Structure
G-coupled receptor family 1, membrane protein, 45 kD
Distribution

Subset of T cells, monocytes

Function
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1
Ligand/Receptor
MIP-1α, MIP-1ß, RANTES
Cell Type
Dendritic cells, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Samson M, et al. 1996. Biochemistry 35:3362.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147.
4. Deng H, et al. 1996. Nature 381:661.
5. Lai J, et al. 2003. CVI. 10:1123.
6. Mañes S, et al. 2003. J. Exp. Med. 198:1381.
7. Vaday GG, et al. 2006. Prostate 66:124.

Gene ID
1234 View all products for this Gene ID
UniProt
View information about CD195 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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