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Alexa Fluor® 647 anti-human CD202b (Tie2/Tek) Antibody

Pricing & Availability
Clone
33.1 (Ab33) (See other available formats)
Regulatory Status
RUO
Other Names
Tie2, TEK
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
33dot1_A647_100908.jpg
Human endothelial cell line, HUVEC, stained with 33.1 Alexa Fluor® 647
  • 33dot1_A647_100908.jpg
    Human endothelial cell line, HUVEC, stained with 33.1 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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334209 25 tests $141
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334210 100 tests $293
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Description

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of human Tie2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Peters KG, et al. 1998. Br. J. Cancer. 77:51.
  2. Wong AL, et al. 1997. Circ. Res. 81:567.
  3. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
Product Citations
  1. Kato D, et al. 2020. Elife. :9. PubMed
  2. Sakai D, et al. 2018. JOR Spine. 1:e1018. PubMed
  3. Rogacev K, et al. 2015. Nephrol Dial Transplant. 30:143. PubMed
  4. Khan OF, et al. 2018. Sci Adv. 4:eaar8409. PubMed
  5. Wangler S, et al. 2019. Spine (Phila Pa 1976). 44:1613. PubMed
  6. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
RRID
AB_2203206 (BioLegend Cat. No. 334209)
AB_2203206 (BioLegend Cat. No. 334210)

Antigen Details

Structure
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution

Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes

Function
Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand/Receptor
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1.De Palma M, et al. 2005. Cancer Cell. 8:211
2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168
3. Hsu HC, et al. 2000. Blood. 96:3757
4. Arai F, et al. 2004. Cell. 118:149

Gene ID
7010 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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