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Alexa Fluor® 647 anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
NAT105 (See other available formats)
Regulatory Status
RUO
Other Names
PD1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NAT105_A647_CD279_PD-1_Antibody_1_111717
Human peripheral blood lymphocytes were stained with CD3 PE and CD279 (clone NAT105) Alexa Flour® 647 (top), or mouse IgG1, κ Alexa Flour® 647 isotype control (bottom).
  • NAT105_A647_CD279_PD-1_Antibody_1_111717
    Human peripheral blood lymphocytes were stained with CD3 PE and CD279 (clone NAT105) Alexa Flour® 647 (top), or mouse IgG1, κ Alexa Flour® 647 isotype control (bottom).
  • NAT105_A647_CD279_PD-1_Antibody_2_111717
  • NAT105_A647_CD279_PD-1_Antibody_3_111717
    PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD279 (clone NAT105) Alexa Flour® 647 (filled histogram) or mouse IgG1, κ Alexa Flour® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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367419 25 tests $147
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367420 100 tests $335
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
RRID
AB_2721353 (BioLegend Cat. No. 367419)
AB_2721353 (BioLegend Cat. No. 367420)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 11/20/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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