- Clone
- 50C1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![50C1_-Alexa-Fluor-647_CD371_Antibody_032523 50C1_-Alexa-Fluor-647_CD371_Antibody_032523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/50C1_-Alexa-Fluor-647_CD371_Antibody_032523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with anti-human CD371 (CLEC12A) (clone 50C1) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
353621 | 25 tests | $130 | ||||
353622 | 100 tests | $265 |
CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family. It is expressed on monocytes, granulocytes, NK cells, and basophils. Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 293T cells expressing CLEC12A-Flag
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Lahoud MH, et al. 2009. J. Immunol. 182:7587. (FC)
- Lahoud MH, et al. 2011. J. Immunol. 187:842. (FC)
- Neumann K, et al. 2014. Immunity. 40:389. PubMed
- RRID
-
AB_2936772 (BioLegend Cat. No. 353621)
AB_2936772 (BioLegend Cat. No. 353622)
Antigen Details
- Structure
- Type II C-type lectin family member, 265 aa, 30.7 kD
- Distribution
-
Monocytes, granulocytes, NK cells, and basophils
- Function
- Negative regulator of granulocyte and monocyte function
- Interaction
- PTPN6 and PTPN11
- Cell Type
- Basophils, Dendritic cells, Granulocytes, Monocytes, NK cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Lahoud MH, et al. 2009. J. Immunol. 182:7587.
2. Chen CH, et al. 2006. Blood 107:1459.
3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792. - Gene ID
- 160364 View all products for this Gene ID
- UniProt
- View information about CD371 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD371 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD371 (CLEC12A) | 50C1 | FC |
PE anti-human CD371 (CLEC12A) | 50C1 | FC |
APC anti-human CD371 (CLEC12A) | 50C1 | FC |
FITC anti-human CD371 (CLEC12A) | 50C1 | FC |
PE/Cyanine7 anti-human CD371 (CLEC12A) | 50C1 | FC |
PerCP/Cyanine5.5 anti-human CD371 (CLEC12A) | 50C1 | FC |
TotalSeq™-A0853 anti-human CD371 (CLEC12A) | 50C1 | PG |
TotalSeq™-C0853 anti-human CD371 (CLEC12A) | 50C1 | PG |
TotalSeq™-D0853 anti-human CD371 (CLEC12A) | 50C1 | PG |
TotalSeq™-B0853 anti-human CD371 (CLEC12A) | 50C1 | PG |
Alexa Fluor® 647 anti-human CD371 (CLEC12A) | 50C1 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with purified ... -
PE anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with anti-huma... -
APC anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with anti-huma... -
FITC anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with anti-huma... -
PE/Cyanine7 anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with anti-huma... -
PerCP/Cyanine5.5 anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with CD371 (CL... -
TotalSeq™-A0853 anti-human CD371 (CLEC12A)
-
TotalSeq™-C0853 anti-human CD371 (CLEC12A)
-
TotalSeq™-D0853 anti-human CD371 (CLEC12A)
-
TotalSeq™-B0853 anti-human CD371 (CLEC12A)
-
Alexa Fluor® 647 anti-human CD371 (CLEC12A)
Human peripheral blood monocytes were stained with anti-huma...
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