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Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) Antibody

Pricing & Availability
Clone
A20004B (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A20004B_A647_CDC2_Antibody_030623
HeLa cells untreated (negative control, open histogram) or treated with 4mM Hydroxyurea (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) (clone A20004B), or with Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for treated and untreated cells) (Cat. No. 400330).
  • A20004B_A647_CDC2_Antibody_030623
    HeLa cells untreated (negative control, open histogram) or treated with 4mM Hydroxyurea (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) (clone A20004B), or with Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for treated and untreated cells) (Cat. No. 400330).
Compare all formats See Alexa Fluor® 647 spectral data
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947405 25 tests $198
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947406 100 tests $469
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Description

Cyclin-dependent kinase 1 (Cdk1), also known as cell division control protein 2 (cdc2), is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, cdc2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-cdc2 complex is activated during G2 and early mitosis by cdc25-mediated dephosphorylation. During mitosis, cdc2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. cdc2 can function as a substitute for other cdc family members and drive the progression of the cell cycle in the event of their loss. cdc2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue.

This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.

RRID
AB_2936756 (BioLegend Cat. No. 947405)
AB_2936756 (BioLegend Cat. No. 947406)

Antigen Details

Structure
cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
Distribution

Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm

Function
Serine/threonine-protein kinase/ Cell Cycle
Interaction
Cyclin B1, Cyclin A, PKMYT1 (MYT1)
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91.
  2. Diril M, et al. 2012. PNAS. 109:3826-31.
  3. Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147.
  4. Liao H, et al. 2017. Oncotarget. 8:90662-73.
  5. Prevo R, et al. 2018. Cell Cycle. 17:1513-23.
Gene ID
983 View all products for this Gene ID
UniProt
View information about cdc2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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