- Clone
- B-D48 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PRF1, P1, PFP, HPLH2
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![B-D48_A647_Perforin_Antibody_101018 B-D48_A647_Perforin_Antibody_101018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/B-D48_A647_Perforin_Antibody_101018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were surface stained with CD8 PE, fixed with fixation buffer, permeabilized with intracellular staining permeabilization wash buffer and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor® 647 (left), or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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353321 | 25 tests | $141 | ||||
353322 | 100 tests | $352 |
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Product Citations
-
- RRID
-
AB_2783256 (BioLegend Cat. No. 353321)
AB_2783257 (BioLegend Cat. No. 353322)
Antigen Details
- Structure
- 70 kD
- Distribution
-
CTL, NK (cytoplasmic granules)
- Function
- Mediates targeted cell lysis.
- Cell Type
- NK cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735. - Gene ID
- 5551 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Related FAQs
Other Formats
View All Perforin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Brilliant Violet 421™ anti-human Perforin | B-D48 | ICFC |
PE anti-human Perforin | B-D48 | ICFC |
Pacific Blue™ anti-human Perforin | B-D48 | ICFC |
APC anti-human Perforin | B-D48 | ICFC |
PerCP/Cyanine5.5 anti-human Perforin | B-D48 | ICFC |
PE/Cyanine7 anti-human Perforin | B-D48 | ICFC |
FITC anti-human Perforin | B-D48 | ICFC |
Alexa Fluor® 700 anti-human Perforin | B-D48 | ICFC |
APC/Fire™ 750 anti-human Perforin | B-D48 | ICFC |
Alexa Fluor® 488 anti-human Perforin | B-D48 | ICFC |
Alexa Fluor® 647 anti-human Perforin | B-D48 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 421™ anti-human Perforin
Human peripheral lymphocytes were surface stained with CD56 ... -
PE anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Pacific Blue™ anti-human Perforin
Human peripheral lymphocytes were surface stained with CD56 ... -
APC anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
PerCP/Cyanine5.5 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine7 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
FITC anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Alexa Fluor® 700 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
APC/Fire™ 750 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Alexa Fluor® 488 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Alexa Fluor® 647 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with...
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