Loading...

Alexa Fluor® 647 anti-human Podoplanin Antibody

Pricing & Availability
Clone
LpMab-21 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
LpMab-21_A647_Podoplanin_Antibody_122118.png
Human LN319 cell line was stained with anti-human podoplanin (clone LpMab-21) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
  • LpMab-21_A647_Podoplanin_Antibody_122118.png
    Human LN319 cell line was stained with anti-human podoplanin (clone LpMab-21) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
  • LpMab-21_A647_Podoplanin_Antibody_2_072519
    Human paraffin-embedded normal breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% Triton-X 100 and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-human podoplanin (clone Lpmab-21, red) and Alexa Fluor® 594 anti-vimentin (clone O91D3, green) at 4°C overnight. Nuclei were counterstained with DAPI (Blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
395003 25 tests $159
Check Availability


Need larger quantities of this item?
Request Bulk Quote
395004 100 tests $358
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human podoplanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kaneko MK, et al. 2017. Cancer Med. 6(2):382-396 (IHC-P, FC)
Product Citations
  1. Tuohinto K, et al. 2023. PLoS Pathog. 19:e1010753. PubMed
  2. Sauler M, et al. 2022. Nat Commun. 13:494. PubMed
RRID
AB_2783309 (BioLegend Cat. No. 395003)
AB_2783309 (BioLegend Cat. No. 395004)

Antigen Details

Structure
40-43 kD type-I transmembrane sialomucin-type glycoprotein
Distribution

Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells

Function
Lymphatic vessel formation, tumor metastasis, platelet aggregation
Ligand/Receptor
CLEC2
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Raica M, et al. 2008. Anticancer Res. 28:2997.
  2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
  3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
  4. Kato Y, et al. 2003. J. Biol. Chem. 278:51599.
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account