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Alexa Fluor® 647 anti-human TRA-1-81 Antibody

Pricing & Availability
Clone
TRA-1-81 (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin
Isotype
Mouse IgM, κ
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Product Citations
publications
Tra-1-81_A647_102108.jpg
NCCIT (human teratocarcinoma cell line) stained with TRA-1-81 Alexa Fluor® 647
  • Tra-1-81_A647_102108.jpg
    NCCIT (human teratocarcinoma cell line) stained with TRA-1-81 Alexa Fluor® 647
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330705 25 tests $165
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330706 100 tests $307
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Description

The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep cl.2A6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrews PW, et al. 1984. Hybridoma. 3:347.
  2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
Product Citations
  1. Leavens KF, et al. 2020. Stem Cell Res. 50:102112. PubMed
  2. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  3. Bozaoglu K, et al. 2020. Stem Cell Res. 49:102013. PubMed
  4. Gagne AL et al. 2018. Stem cell research. 31:157-160 . PubMed
  5. Vlahos K et al. 2018. Stem cell research. 1723:40:00 . PubMed
  6. Howden S et al. 2019. Stem Cell Res. 1728:53:00 . PubMed
  7. Bozaoglu K, et al. 2020. Stem Cell Research. 39:101516.. PubMed
  8. Hosseini Far H et al. 2019. Stem Cell Res. 1727:49:00 . PubMed
  9. Maguire JA et al. 2019. Stem Cell Res. 1723:21:00 . PubMed
  10. V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed
  11. Tian P, et al. 2022. Front Cell Dev Biol. 10:835321. PubMed
RRID
AB_1089242 (BioLegend Cat. No. 330705)
AB_1089242 (BioLegend Cat. No. 330706)

Antigen Details

Structure
Mucin-like protein, CD34 family member, 200-240 kD
Distribution

embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells

Function
Anti-adhesion
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Antigen References

1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about TRA-1-81 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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