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Alexa Fluor® 647 anti-mouse CD74 (CLIP) Antibody

Pricing & Availability
Clone
In1/CD74 (See other available formats)
Regulatory Status
RUO
Other Names
CLIP, DHLAG, HLADG
Isotype
Rat IgG2b, κ
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Product Citations
publications
In1slashCD74_A647_CD74_Antibody_1_093016
C57BL/6 splenocytes were stained with CD19 PE and CD74 (clone In1/CD74) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • In1slashCD74_A647_CD74_Antibody_1_093016
    C57BL/6 splenocytes were stained with CD19 PE and CD74 (clone In1/CD74) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • In1slashCD74_A647_CD74_Antibody_2_093016
  • In1slashCD74_A647_CD74_IHC_070518
    One week 4T1 cells (mouse breast cancer cell line) induced BALB/c frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD3ε (clone 500A2) Alexa Fluor® 594 (blue), CD74 (clone In1/CD74) Alexa Fluor® 647 (red) and B220 (clone RA3-6B2) Alexa Fluor® 488 (green) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
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151003 25 µg $159
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151004 100 µg $382
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Description

CD74, also known as CLIP, is a 33.5kD homotrimer and functions as a MHC class II chaperone for exogenous peptides and other MHC class II molecules. CD74 binds MIF and regulates innate and adaptive immunity through CD44 signaling. It is found primarily on B-cells, monocytes, macrophages and dendritic cells, but can also be found on activated T-cells, endothelial and epithelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry1, immunoprecipitation3, and western blotting4.

Application References

(PubMed link indicates BioLegend citation)
  1. Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
  2. Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
  3. Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
  4. Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Radtke D, et al. 2022. Elife. 11: . PubMed
  3. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
  4. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  5. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  6. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID
AB_2632608 (BioLegend Cat. No. 151003)
AB_2632608 (BioLegend Cat. No. 151004)

Antigen Details

Structure
33.5 kD and homotrimer.
Distribution

B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.

Function
MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
Interaction
MHC class II molecules and peptides.
Ligand/Receptor
Macrophage migration inhibitory factor (MIF).
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.

Gene ID
16149 View all products for this Gene ID
UniProt
View information about CD74 on UniProt.org
Go To Top Version: 1    Revision Date: 10/03/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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