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Alexa Fluor® 647 anti-mouse/human A2B5 Antibody

Pricing & Availability
Clone
105 (See other available formats)
Regulatory Status
RUO
Other Names
F12A2B5, 105
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
105_A647_A2B5_Antibody_FC_103015
Insulinoma cell line, Beta-TC-6, was stained with A2B5 (clone 105) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).
  • 105_A647_A2B5_Antibody_FC_103015
    Insulinoma cell line, Beta-TC-6, was stained with A2B5 (clone 105) Alexa Fluor® 647 (filled histogram) or mouse IgM, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
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150703 25 µg $159
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150704 100 µg $370
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Description

The 105/A2B5 antibody recognizes a membrane protein that is expressed by chicken retinal cells, neuronal cells, ganglia, and spinal cord. In combination with other markers, this antibody can be used to differentiate between type 1 and type 2 astrocytes. The antibody recognizes polysialogangliosides and can be used to monitor glial cell development. A2B5 antigens are expressed by a variety of tumors including glioblastomas. Tumors expressing A2B5 antigen exhibit higher recurrence. The A2B5 antibody can also be used to identify progenitor cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Embryonic chicken retinal cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.1,2

Application References

(PubMed link indicates BioLegend citation)
  1. Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)
  2. Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)
  3. Russell TR, et al. 1984. Cytometry 5:539. (FC)
RRID
AB_2566394 (BioLegend Cat. No. 150703)
AB_2566394 (BioLegend Cat. No. 150704)

Antigen Details

Distribution
Developing thymic epithelial cells, oligodendrocyte progenitors, neuroendocrine, embryonic neonatal tissue, astrocytoma, glioma, and squamous cell carcinoma.
Cell Type
Epithelial cells, Oligodendrocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Baracskay KL, et al. 2007. GLIA 55:1001.
2. Raff MC, et al. 1983. J. Neurosci. 6:1289.
3. Fredman P, et al. 1984. Arch. Biochem. Biophys. 2:661.
4. Xia CL, et al. 2003. Int. J. Oncol. 23:353.
5. Tchoghandjian A, et al. Brain Pathol. 20:211.

Gene ID
NA
UniProt
View information about A2B5 on UniProt.org
Go To Top Version: 1    Revision Date: 11/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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