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Alexa Fluor® 647 anti-mouse/human Bcl-6 Antibody

Pricing & Availability
Clone
IG191E/A8 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell lymphoma 6, BCL-5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
IG191E-A8_A647_Bcl6_Antibody_1_102224
C57BL/6 lymph node cells were immunized with CFA for 12 days, fixed, and permeabilized with BioLegend Nuclear Factor Fixation and Permeabilization Buffer Set, then stained with CD19 FITC, IgD PerCP/Cyanine5.5, and Bcl-6 (clone IG191E/A8) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right). Dot plots are gated on CD19+ lymphocytes.
  • IG191E-A8_A647_Bcl6_Antibody_1_102224
    C57BL/6 lymph node cells were immunized with CFA for 12 days, fixed, and permeabilized with BioLegend Nuclear Factor Fixation and Permeabilization Buffer Set, then stained with CD19 FITC, IgD PerCP/Cyanine5.5, and Bcl-6 (clone IG191E/A8) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right). Dot plots are gated on CD19+ lymphocytes.
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648305 25 tests $147
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648306 100 tests $405
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Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family.  It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Plasmid vector containing cDNA of the Bcl-6 gene
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is also known as GI191E/A8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin fixed, paraffin embedded tissue sections1, Western blotting1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Garcfa JF, et al. 2006. J. Histochem Cytochem. 54:31.
Product Citations
  1. Jiang W, et al. 2021. Sci Rep. 11:1864. PubMed
  2. Barber-Axthelm IM, et al. 2023. iScience. 26:106269. PubMed
  3. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  4. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
RRID
AB_2565298 (BioLegend Cat. No. 648305)
AB_2565298 (BioLegend Cat. No. 648306)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Ye BH, et al. 1993. Science 262:747.
2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262.
3. Onizuka T, et al. 1995. Blood 86:28.
4. Johnston RJ, et al. 2009. Science 325:1006.
5. Kitano M, et al. 2011. Immunity 34:961.
6. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
7. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID 12053 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/22/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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