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Alexa Fluor® 647 anti-mouse/human Mac-2 (Galectin-3) Antibody

Pricing & Availability
Clone
M3/38 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Rat IgG2a, κ
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Product Citations
publications
M3slash38_A647_Mac2_Antibody_1_022318
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with M3/38 Alexa Fluor® 647
  • M3slash38_A647_Mac2_Antibody_1_022318
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with M3/38 Alexa Fluor® 647
  • M3slash38_A647_Mac2_Antibody_2_022318
    Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of anti-human Mac2 (clone M3/38) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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125408 100 µg $265
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Description

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against galectin-3 of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.

Application References

(PubMed link indicates BioLegend citation)
  1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
  2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
  3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
  4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
  5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
  6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
  7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
  8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
  9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
  10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
Product Citations
  1. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  2. Herbst S, et al. 2020. EMBO J. 39:e104494. PubMed
  3. Kouo T, et al. 2015. Cancer Immunol Res. 3:412. PubMed
  4. Riggs JB, et al. 2021. Front Microbiol. 12:656979. PubMed
  5. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  6. Miller A et al. 2017. Cell metabolism. 26(5):788-800 . PubMed
  7. Popa S, et al. 2020. Wellcome Open Res. 0.249305556. PubMed
RRID
AB_1186110 (BioLegend Cat. No. 125408)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.

Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 11/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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