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- Clone
- 8.48 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor Vb10
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
SJL splenocytes stained with CD3 (145-2C11) PE and 8.48 Alexa Fluor® 647
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 128903 | 25 µg | $141 | ||||
This antibody reacts with Vb10 TCR of mice having the a haplotype (SJL, C57BR, SWR, C57L), but not with TCRb (e.g., AKR, BALB/c C3H, C57BL, DBA/1, DBA/2) or TCRc haplotypes (e.g., RIII).
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 1B6 Rag2 knockout transgenic T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_1236445 (BioLegend Cat. No. 128903)
Antigen Details
- Structure
- TCR consists of alpha and beta chain. This antibody recognizes Vb 10 of TCR a haplotype (e.g. SJL, C57L, SWR and C57BR).
- Distribution
-
Positive on a subset of alpha/beta T cells from mice of TCR a haplotype.
- Ligand/Receptor
- MHC/peptide
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Necker A, et al. 1991. Eur. J. Immunol. 21:3035
- Gene ID
- 100124660 View all products for this Gene ID
- UniProt
- View information about TCR Vbeta10 on UniProt.org
Related FAQs
Other Formats
View All TCR Vβ10 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Alexa Fluor® 647 anti-mouse TCR Vβ10 (a haplotype) | 8.48 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-mouse TCR Vβ10 (a haplotype)
SJL splenocytes stained with CD3 (145-2C11) PE and 8.48 Alex...
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