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Alexa Fluor® 647 anti-rat IgG2a Antibody

Pricing & Availability
Clone
MRG2a-83 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Mouse IgG
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Product Citations
publications
MRG2a-85_A647_IgG2b_Antibody_FC_071218
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 647 .
  • MRG2a-85_A647_IgG2b_Antibody_FC_071218
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 647 .
  • MRG2b-85_A647_IgG2b_Antibody_IHC_100217
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD8a (clone 53-6.7) purified and 10 µg/ml of CD20 (clone SA275A11) Brilliant Violet 421™ (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2a (Clone MRG2a-83) Alexa Fluor® 647 (red) for 2 hours at room temperature. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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407511 25 µg $89
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407512 100 µg $194
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Description

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
Product Citations
  1. Peymanfar Y, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  3. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  4. Park JY, et al. 2019. Theranostics. 9:3398. PubMed
RRID
AB_2716139 (BioLegend Cat. No. 407511)
AB_2716139 (BioLegend Cat. No. 407512)
Go To Top Version: 0    Revision Date: 10/03/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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