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Alexa Fluor® 647 anti-rat TCR α/β Antibody

Pricing & Availability
Clone
R73 (See other available formats)
Regulatory Status
RUO
Other Names
TCR α/β, α/β TCR
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
R73_Alx647_021308
LOU rat splenocytes stained with R73 Alexa Fluor® 647
  • R73_Alx647_021308
    LOU rat splenocytes stained with R73 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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201116 100 µg $229
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Description

TCR α and β are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most peripheral T cells, intestinal epithelial lymphocytes, and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The TCR α/β recognizes peptide bound to MHC class I and II.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat T cell blasts & erythrocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, immunohistochemical staining of acetone-fixed frozen sections1, in vitro activation of α/β TCR+ T cells 1,2 and in vivo depletion2 of α/β TCR+ T cells . The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 201112).

Application References

(PubMed link indicates BioLegend citation)
  1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
  2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
  3. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
  4. Yoshino N, et al. 2000. Exp. Anim. 49:97.
Product Citations
  1. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  2. Stoji?-Vukani? Z, et al. 2017. Molecular Neurobiology. 10.1007/s12035-017-0595-2. PubMed
  3. Winkler A, et al. 2021. J Clin Invest. 131:. PubMed
  4. Nacka-Aleksić M, et al. 2015. Brain Behav Immun. . PubMed
  5. Schläger C, et al. 2016. Nature. 530:349-353. PubMed
  6. Djikić J, et al. 2014. Exp Gerontol. 58:179. PubMed
RRID
AB_572055 (BioLegend Cat. No. 201116)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR-α subunit, 40 and 46 kD
Distribution

Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes

Function
Antigen recognition, T cell activation/responses
Ligand/Receptor
Peptide bound-MHC class I and II
Cell Type
Lymphocytes, T cells, Thymocytes
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Fangmann J, et al. 1991. Eur. J. Immunol. 21:753.
3. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
4. Peiters RH, et al. 1995. Biochem. Biophys. Res. Commun. 214:552.
5. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.

Gene ID
24820 View all products for this Gene ID 290071 View all products for this Gene ID
UniProt
View information about TCR alphabeta on UniProt.org
Go To Top Version: 3    Revision Date: 11/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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