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Alexa Fluor® 647 anti-SHIP-1 Antibody

Pricing & Availability
Clone
P1C1-A5 (See other available formats)
Regulatory Status
RUO
Other Names
Inositol Polyphosphate-5-Phosphatase, SIP-145, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1C1-A5_AF647_SHIP-1_Antibody_FC_012914.jpg
C57BL/6 mouse bone marrow cells were stimulated with or without LPS for 48 hours, then fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-1 (clone P1C1-A5) Alexa Fluor® 647 (filled histogram for LPS stimulated, open histogram with purple solid line for unstimulated) or mouse IgG1, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated). Data shown was gated on myeloid cell population.
  • P1C1-A5_AF647_SHIP-1_Antibody_FC_012914.jpg
    C57BL/6 mouse bone marrow cells were stimulated with or without LPS for 48 hours, then fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-1 (clone P1C1-A5) Alexa Fluor® 647 (filled histogram for LPS stimulated, open histogram with purple solid line for unstimulated) or mouse IgG1, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated). Data shown was gated on myeloid cell population.
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656607 25 µg $176
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656608 100 µg $399
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Description

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes key signaling lipid PI(3,4,5)P(3) to PI(3,4)P(2). SHIP-1 is located in the cytoplasm and is recruited to the cell surface upon receptor ligation. It plays critical roles in hematopoietic cell proliferation, activation, differentiation, and survival by regulating PI3K and Akt pathways. Aberrant regulation of PI3K signaling can induce autoimmune diseases and cancer formation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human SHIP fusion protein in CFA/IFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Specific to human SHIP-1 and cross reactive with mouse SHIP-1.

Product Citations
  1. Pan L, et al. 2020. Elife. :9. PubMed
RRID
AB_2563145 (BioLegend Cat. No. 656607)
AB_2563145 (BioLegend Cat. No. 656608)

Antigen Details

Structure
1189 or 1188 amino acids, predicted molecular weight of 150 kD with the characteristic N-terminal SH2 domain, inositol phosphatase domain, and C-terminal protein interaction domains
Distribution
Cytoplasm and membrane; translocates to the plasma membrane when activated
Function
Negative regulator of hematopoietic cell proliferation and survival
Interaction
Phosphatidylinositol-3,4,5-triphosphate
Ligand/Receptor
SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150, PTPN11 FCGR2A FCGR2B, FCGR3, SRC and TEC et al.
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Condé C, et al. 2011. Biochem. Pharmacol. 82:1320.
2. Parry RV, et al. 2010. Biochim Biophys Acta. 1804:592.
3. Ghansah T, et al. 2012. Oncoimmunology. 1:984.
4. Banh C, et al. 2012. Blood 120:4583.
5. Blunt MD, et al. 2012. Front. Immunol. 3:226.

Gene ID
3635 View all products for this Gene ID
UniProt
View information about SHIP-1 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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