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Alexa Fluor® 647 anti-SOX2 Antibody

Pricing & Availability
Clone
14A6A34 (See other available formats)
Regulatory Status
RUO
Other Names
SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_14A6A36_AF647_SOX2_Antibody_ICC_052419
NCCIT cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 at room temperature for 10 minutes, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with anti-human SOX2 (clone 14A6A34) Alexa Fluor® 647 (red) overnight at 4°C. F-actin was stained with Phalloidin Alexa Fluor® 488 (green) and nuclei were counterstained with DAPI (blue).
  • 1_14A6A36_AF647_SOX2_Antibody_ICC_052419
    NCCIT cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 at room temperature for 10 minutes, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with anti-human SOX2 (clone 14A6A34) Alexa Fluor® 647 (red) overnight at 4°C. F-actin was stained with Phalloidin Alexa Fluor® 488 (green) and nuclei were counterstained with DAPI (blue).
  • 2_14A6A34_A647_SOX2_Antibody_ICFC_081414
    Human embryonic carcinoma NCCIT cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with SOX2 (clone 14A6A34) AF647 (filled histogram) or mouse IgG1, κ AF647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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656108 100 µg $311
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Description

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SOX recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Pal D, et al. 2022. Cell Rep Med. 3:100717. PubMed
  2. Shrestha R, et al. 2020. Stem Cell Research. 41:101590.. PubMed
  3. Shrestha R, et al. 2019. Cells. 8:. PubMed
RRID
AB_2563681 (BioLegend Cat. No. 656108)

Antigen Details

Structure
317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution

Nucleus

Function
Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction
Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.

Gene ID
6657 View all products for this Gene ID
UniProt
View information about SOX2 on UniProt.org
Go To Top Version: 1    Revision Date: 08/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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