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Alexa Fluor® 647 anti-STAT4 Phospho (Tyr693) Antibody

Pricing & Availability
Clone
A19016A (See other available formats)
Regulatory Status
RUO
Other Names
Signal Transducer and Activator Of Transcription 4, SLEB11, Signal Transducer and Activator of Transcription 4 Variant 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
A19016A_Alexa-Fluor-647_Stat4_Antibody_013023
NK-92 cells untreated (negative control, open histogram), or treated with hIL-2 (100 ng/mL) and IFNα2 (100 ng/mL) (positive control, solid histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Stat4 Phospho (Tyr693) (clone A19016A), or Alexa Fluor® 647 Mouse IgG1, κ isotype control (open histogram, dashed line)(representative for both untreated and treated NK-92 cells) (Cat No. 400155).
  • A19016A_Alexa-Fluor-647_Stat4_Antibody_013023
    NK-92 cells untreated (negative control, open histogram), or treated with hIL-2 (100 ng/mL) and IFNα2 (100 ng/mL) (positive control, solid histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Stat4 Phospho (Tyr693) (clone A19016A), or Alexa Fluor® 647 Mouse IgG1, κ isotype control (open histogram, dashed line)(representative for both untreated and treated NK-92 cells) (Cat No. 400155).
  • A19016A_Alexa-Fluor-647_Stat4_Antibody_2_013023
    PBMC pre-treated overnight with cell activation cocktail (1:2500)(Cat. No. 423301). They were then treated with (positive control, solid histogram) or without (negative control, open histogram) hIL-2 (100 ng/mL) and IFNα2 (100 ng/mL) for 30 min, fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Stat4 Phospho (Tyr 693) (clone A19016A), or Alexa Fluor® 647 Mouse IgG1, κ isotype control (open histogram, dashed line)(representative for both untreated and treated PBMC) (Cat. No. 400155).
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941203 25 tests $198
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941204 100 tests $469
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Description

The signal transducer and activator of transcription (STAT) family of transcription factors are DNA-binding proteins required for transcriptional regulation of several cellular and physiological processes in response to cytokine and growth factor stimulation. Inactive STAT proteins are present as cytosolic monomers. C-terminal tyrosine phosphorylation by upstream Janus kinases results in STAT dimerization, translocation to the nucleus, and increased affinity for response elements found in the regulatory promoter of STAT target genes.

In contrast to most other members of the STAT family, STAT4 expression is restricted to hematopoietic cells and responds to fewer cytokines, such as IL-12 and IL-2. STAT4, which requires phosphorylation at tyrosine 693 for activation, plays a pivotal role in the differentiation and proliferation of Th1 and Th17 cells, both of which are crucial effectors of chronic autoimmune disorders. In natural killer cells, STAT4 is required for normal development, maturation, and activation. Single nucleotide polymorphisms of STAT4 are implicated in the pathogenesis of systemic lupus erythematosis and rheumatoid arthritis, and are linked with increased risk of hepatocellular carcinoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody is predicted to recognize mouse STAT4 phosphorylated at tyrosine 694 and rat STAT4 phosphorylated at tyrosine 693 due to complete sequence homology between the immunizing peptide and the respective murine STAT4 orthologs.

RRID
AB_2936723 (BioLegend Cat. No. 941203)
AB_2936723 (BioLegend Cat. No. 941204)

Antigen Details

Structure
STAT4 is a 748 amino acid protein with predicted molecular weight of 86 kD.
Distribution

Hematopoietic cells/Nucleocytoplasmic localization

Function
Transcription factor, cell signaling
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References

Trinchieri G, et al. 2003. Nat. Rev. Immunol. 3:133.
Remmers EF, et al. 2007. N. Engl J. Med. 357:977.
Mukasa R, et al. 2010. Immunity. 32:616
Rapp M, et al. 2017. Sci. Immunol. 2:3296.

Gene ID
6775 View all products for this Gene ID
UniProt
View information about STAT4 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/30/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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