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Alexa Fluor® 700 anti-human CD8a Antibody

Pricing & Availability
Clone
HIT8a (See other available formats)
Regulatory Status
RUO
Workshop
V CD08.10
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIT8a_Alx700_022008
Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 700
  • HIT8a_Alx700_022008
    Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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300919 25 µg $124
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300920 100 µg $265
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Common Marmoset
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
  5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
  6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
Product Citations
  1. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  2. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  3. Rosenberg JM, et al. 2022. Med (N Y). 3:42. PubMed
  4. Wang F, et al. 2023. Sci Adv. 9:eadf5464. PubMed
  5. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  6. Trzupek D, et al. 2022. Wellcome Open Res. 6:149. PubMed
  7. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  8. Kinjyo I, et al. 2010. J Immunol. 185:7151. PubMed
  9. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  10. Du L, et al. 2021. Front Mol Biosci. 8:675179. PubMed
  11. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  12. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  13. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  14. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  15. Meng Q, et al. 2016. Arch Virol. 161: 181-187. PubMed
  16. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  17. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  18. Westera L, et al. 2017. Clin Transl Gastroenterol. 8:e126. PubMed
  19. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  20. Teirlinck A, et al. 2011. PLoS One. 7:e1002389. PubMed
  21. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
  22. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
  23. Wilmes S, et al. 2021. Elife. 10:. PubMed
  24. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  25. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  26. Wells DK, et al. 2020. Cell. 183:818. PubMed
  27. Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
  28. Li L, et al. 2022. JCI Insight. 7:. PubMed
  29. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  30. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  31. Tosch C, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0274-x. PubMed
RRID
AB_528884 (BioLegend Cat. No. 300919)
AB_528884 (BioLegend Cat. No. 300920)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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