Alexa Fluor 700 anti-mouse Ig light chain kappa

Pricing & Availability

Clone: RMK-45 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin light chain kappa
Isotype: Rat IgG
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Product Citations: publications

RMK-45_AF700_IgK_Antibody_FC_061714 — C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).

Compare all formats See Alexa Fluor® 700 spectral data

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409507

25 µg

$124

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409508

100 µg

$323

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Description

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.
RRID: AB_2563582 (BioLegend Cat. No. 409507)
AB_2563582 (BioLegend Cat. No. 409508)

Antigen Details

Structure: Immunoglobulin family
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References: 1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.
Gene ID: 243469 View all products for this Gene ID
UniProt: View information about Ig light chain kappa on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Other Formats

View All Ig light chain κ Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ig light chain κ	RMK-45	FC,ELISA
APC/Cyanine7 anti-mouse Ig light chain κ	RMK-45	FC
PE anti-mouse Ig light chain κ	RMK-45	FC
Alexa Fluor® 700 anti-mouse Ig light chain κ	RMK-45	FC
FITC anti-mouse Ig light chain κ	RMK-45	FC

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Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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