Alexa Fluor® 594 anti-human CD174 Antibody anti-CD174

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Clone: NCRC37 (See other available formats)
Regulatory Status: RUO
Other Names: SC104
Isotype: Mouse IgG1, κ
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NCRC37_A594_CD174_Antibody_040218 — Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0 M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes permeabilized with 0.5% TritonX-100 in PBS for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 goat anti-Vimentin (Clone O91D3) antibody (green) and Alexa Fluor® 594 anti-human CD174 (Clone NCRC37) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.

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392603

25 µg

$159

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392604

100 µg

$382

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Description

CD174 is also known as Lewis Y, Fucosyltransferase 3, Galactoside 3(4)-L-fucosyltransferase which is encoded by FUT3 gene. FUT3 protein has 361a.a., around 39.7 kD and is highly expressed on duodenum and small intestine. The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Tissue cultured colorectal tumor cell line (C170, C146, COLO205, and JW)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID: AB_2728418 (BioLegend Cat. No. 392603)
AB_2728418 (BioLegend Cat. No. 392604)

Antigen Details

Distribution: Highly expressed on glandular cells of duodenum and small intestine.
Function: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme.
Biology Area: Cancer Biomarkers, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Durrant, LG. et al. 2006. Cancer Res. 66:5901-9
Gene ID: 2525 View all products for this Gene ID
UniProt: View information about CD174 on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All CD174 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD174	NCRC37	FC,IHC-P
Alexa Fluor® 594 anti-human CD174	NCRC37	IHC-P
Alexa Fluor® 647 anti-human CD174	NCRC37	IHC-P,FC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 04/02/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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