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Alexa Fluor® 647 anti-NOS3 (eNOS) Antibody

Pricing & Availability
Clone
W22110B (See other available formats)
Regulatory Status
RUO
Other Names
Nitric oxide synthase 3 (NOS3), Endothelial Nos (eNos), constitutive NOS (cNOS)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22110B_AF647_NOS3_Antibody_1_030325
IHC staining with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W22110B) was performed on formalin-fixed paraffin-embedded human endometrium tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 400626) (panel A) or with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W22110B) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and C). Scale Bar: 50 µm
  • W22110B_AF647_NOS3_Antibody_1_030325
    IHC staining with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W22110B) was performed on formalin-fixed paraffin-embedded human endometrium tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 400626) (panel A) or with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W22110B) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and C). Scale Bar: 50 µm
  • W22110B_AF647_NOS3_Antibody_2_030325
    EA.hy926 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W22110B) or Alexa Fluor® 647 Rat IgG2b, κ Isotype Control Antibody (open histogram, dashed line) (Cat No. 400626).
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626753 25 µg $180
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Description

Nitric oxide synthase 3 (NOS3), also commonly known as endothelial nitric oxide synthase (eNOS), is a critical enzyme primarily found in the endothelial cells lining the blood vessels. Its main function is to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a signaling molecule with profound effects on vascular health and overall physiology, regulating blood vessel tone and diameter in response to various physiological cues including shear stress, neurotransmitters, and hormones. NO relaxes and dilates blood vessels, thereby promoting proper blood flow and reducing blood pressure. Additionally, NOS3-derived NO helps prevent the formation of blood clots, reduces inflammation in the blood vessel walls, and maintains vascular homeostasis, contributing significantly to cardiovascular health. Dysfunction or impaired activity of NOS3 is implicated in various cardiovascular diseases, making it an important target for therapeutic interventions aimed at preserving vascular function.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant KLH-conjugated peptide fragment of human NOS3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

ICFC - Verifed
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

Antigen Details

Structure
NOS3 is a 1205 amino acid protein with a predicted molecular weight of 133.3 kD.
Distribution

Cytoplasmic, vascular endothelial cells

Function
Enzyme, nitric oxide (NO) synthesis from L-arginine. Transcriptionally regulated by SP1, SP3, ETS-1, YY1.
Interaction
NOS3 is regulated via binding of its cofactor BH4, which is required to efficiently produce NO.
Cell Type
Endothelial cells
Biology Area
Cardiovascular Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Mineo C and Shaul PW. 2012. Adv Exp Med Biol. 729:51-62. 
  2. Hong FF, et al. 2019. Inflamm Res. 68:429-441. 
  3. Siragusa M and Fleming I. 2016. Pfulgers Arch. 468:1125-1137. 
  4. Musicki B and Burnett AL 2006. Exp Bil Med. 231:154-65.
Gene ID
4846 View all products for this Gene ID
UniProt
View information about NOS3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/03/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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