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Anti-E. coli RNA Polymerase ImmunoAffinity Resin

Pricing & Availability
Clone
NT73 (See other available formats)
Regulatory Status
RUO
Other Names
Softag 1
Previously
NeoClone Catalog# S0001
Isotype
Mouse IgG1
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
673601 1 mL $323
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Description

E. coli RNA Core polymerase is useful in studying mechanisms of transcription initiation. E. coli Core polymerase purified with Softag 4 may be free of sigma factors found in holoenzyme.

Product Details
Technical data sheet

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.
Application

IP - Quality tested
Recommended Usage

Resin binding capacity is greater than 0.15 mg/mL (based on 28kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.

Application Notes

This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 1. Softag 1 is an immunoaffinity system appropriate for purifying tagged proteins from eukaryotic expression sources. The epitope (SLAELLNAGLGGS) is derived from a subunit of prokaryotic RNAP.

Application References

(PubMed link indicates BioLegend citation)

Previously item# S0001 from Neoclone.

  1. Thompson NE, et al. 2003. Anal. Biochem. 323:171.
RRID
AB_3105969 (BioLegend Cat. No. 673601)

Related FAQs

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Go To Top Version: 4    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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