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APC anti-human CD158e1 (KIR3DL1, NKB1) Antibody

Pricing & Availability
Clone
S22024B (See other available formats)
Regulatory Status
RUO
Other Names
CD158e, Killer Cell Immunoglobulin-Like Receptor 3DL1, NKAT3, NKB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S22024B_APC_CD158e1_Antibody_1_021125
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) Pacific Blue™ and anti-human CD158e1 (KIR3DL1, NKB1) (clone S22024B) APC (left) or mouse IgG1, κ APC isotype control (right).
  • S22024B_APC_CD158e1_Antibody_1_021125
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) Pacific Blue™ and anti-human CD158e1 (KIR3DL1, NKB1) (clone S22024B) APC (left) or mouse IgG1, κ APC isotype control (right).
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389503 25 tests $100
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389504 100 tests $240
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158e is a 50 kD three extracellular Ig domain glycoprotein that displays a long cytoplasmic tail with two ITIM motifs. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR3DL1 has been reported to bind to a number of different MHC class molecules that contain the HLA Bw4 epitope and CD8aa.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KIR3DL1-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S22024B does not block the binding of clone DX9 on target cells.

Based on in-house testing on different KIR-expressing transfectants, clone S22024B does not interact with other KIR protein family members, including CD158e2.

Antigen Details

Structure
50 kD three Ig-like extracellular domain glycoprotein
Distribution

Subset of NK cells, subset of T Cells

Function
NK Cell regulation
Ligand/Receptor
MHC Class 1 molecules
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Lanier LL, 2005. Annu Rev Immunol. 23:225-74.
  2. Middleton D, et al. 2010. Immunol. 123:8.
  3. Khakoo SI, et al. 2002. J Exp Med. 196:911-21.
Gene ID
3811 View all products for this Gene ID
UniProt
View information about CD158e1 on UniProt.org
Go To Top Version: 1    Revision Date: 02/11/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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