- Clone
- QA21A13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BLR1, MDR15
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA21A13_APC_CD185_Antibody_1_121323 QA21A13_APC_CD185_Antibody_1_121323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA21A13_APC_CD185_Antibody_1_121323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD185 (CXCR5) recombinant (clone QA21A13) APC (left) or mouse IgG1, κ APC isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
382403 | 25 tests | $145 | ||||
382404 | 100 tests | $370 |
CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Clone QA21A13 can completely block the binding of clone J252D4 on target cells.
- RRID
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AB_3097284 (BioLegend Cat. No. 382403)
AB_3097284 (BioLegend Cat. No. 382404)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions, 42 kD
- Distribution
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Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
- Function
- Mediates cell migration to the B cell follicles in the secondary lymphoid organs
- Ligand/Receptor
- CXCL13
- Cell Type
- B cells, Tfh
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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- Ma CS, et al. 2012. J Exp Med. 209:1241-53.
- León B, et al. 2012. Nat. Immunol. 13:681-90.
- Crotty S. 2011. Annu Rev Immunol. 29:621-63.
- Kerfoot SM, et al. 2011. Immunity. 34:947-60.
- Sáez de Guinoa J, et al. 2011. Blood. 118:1560-9.
- Gene ID
- 643 View all products for this Gene ID
- UniProt
- View information about CD185 on UniProt.org
Other Formats
View All CD185 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD185 (CXCR5) Recombinant Antibody | QA21A13 | FC |
APC anti-human CD185 (CXCR5) Recombinant Antibody | QA21A13 | FC |
PE anti-human CD185 (CXCR5) Recombinant Antibody | QA21A13 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD185 (CXCR5) Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD185 (CXCR5) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD185 (CXCR5) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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