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APC anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
A20050B (See other available formats)
Regulatory Status
RUO
Other Names
CD273, Programmed cell death 1 ligand 2 (PD-L2), B7 dendritic cell molecule (B7-DC), Butyrophilin B7-DC, PDCD1 Ligand 2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A20050B_APC_CD273_Antibody_051623
Human monocyte-derived dendritic cells were stained with anti-human CD273 (B7-DC, PD-L2) (clone A20050B) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • A20050B_APC_CD273_Antibody_051623
    Human monocyte-derived dendritic cells were stained with anti-human CD273 (B7-DC, PD-L2) (clone A20050B) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
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947805 25 tests $145
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947806 100 tests $315
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Description

PD-L2 is a type I transmembrane protein of 273 amino acids, and it is a member of the B7 family. Human PD-L2 has 70% amino acid identity to its mouse orthologue. PD-L2 is the second ligand for PD-1, and its binding to PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L2/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Antibody blocking of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production. Interaction of PD-1 by PD-L1-Ig or PD-L2-Ig fusion protein has an inhibitory effect on proliferation and cytokine production during anti-CD3-mediated stimulation. PD-L2 is expressed in biopsies from human asthmatics and lungs of aeroallergen-treated mice and its expression correlates with the asthma severity. In vivo blockade of PD-L2 reduces the severity of allergen-driven airway hyperresponsiveness (AHR).  Blockage of PD-L2 induces the IL-12 production by DC; IL-12 may limit the severity of allergen-induced AHR antagonizing IL-13. In addition, blockade of PD-1 does not have effect on AHR; therefore, PD-L2 controls asthma severity in a PD-1 independent manner. PD-L2 binds to a second receptor, RGMb (repulsive guidance molecule b) which is expressed in lung interstitial macrophages and alveolar epithelial cells and is a co-receptor for bone morphogenetic proteins BMP2/4. Blockade of the RGMb/PD-L2 interaction significantly impaired the development of respiratory tolerance since the initial T cell expansion is inhibited.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human PD-L2 (B7-DC, CD273)-Fc chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2941639 (BioLegend Cat. No. 947805)
AB_2941639 (BioLegend Cat. No. 947806)

Antigen Details

Structure
Dimer
Distribution

Immature dendritic cells, mature dendritic cells, subset of macrophages, placental endothelium, and medullary thymic epithelial cells

Function
Inhibits T cell mediated proliferation and cytokine production by CD4+ T cells. Regulate T cell responses. PD-L2 expression is up-regulated on antigen-presenting cells by IFN-γ.
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, monocytes
Ligand/Receptor
PD-1 (CD279), RGMb
Cell Type
Antigen-presenting cells, Dendritic cells, Epithelial cells, Macrophages, Plasma cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Latchman Y, et al. 2001. Nat. Immunol. 2:261.
  2. Brown JA, et al. 2003. J. Immunol. 170:1257.
  3. Youngnak P, et al. 2003. Biochem. Biophys. Res. Commun. 307:672.
  4. Singh AK, et al. 2011. Allergy. 66:155.
  5. Lewkowich IP, et al. 2013. Mucosal. Immunol. 6:728.
  6. Xiao Y, et al. 2014. J. Exp. Med. 211:943.
  7. Bardhan K, et al. 2016. Front Immunol. 7:550.
Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/16/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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