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APC anti-human CD301 (CLEC10A) Antibody

Pricing & Availability
Clone
H037G3 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage galactose/Nacetylgalactosamine (GalNAc) specific lectin, MGL, DCASGPR, HML CLECSF13, CLECSF14, HML2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
H037G3_APC_060612
Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • H037G3_APC_060612
    Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
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354705 25 tests $154
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354706 100 tests $367
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Description

CD301, also known as CLEC10A, macrophage galactose/N-acetylgalactosamine (GalNAc) specific lectin (MGL), DCASGPR, and HML, is a 40 kD type II transmembrane glycoprotein, which belongs to the C-type lectin family. Human CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region.  CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. The expression level is upregulated by immunosuppressant dexamethasone. Human CD301 has an exclusive specificity for rare terminal GalNAc structures, which are revealed on the tumor-associated mucin MUC1 and CD45 (RA, RB, and RC but not RO isoforms). This interaction inhibits effector T cell activation and induces their apoptosis. CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant CD301 (extracellular domain)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

H037G3 antibody binding is divalent cation dependent.

Product Citations
  1. Franke AK, et al. 2022. Gene Ther. :. PubMed
  2. Luo X, et al. 2023. Methods Mol Biol. 2618:133. PubMed
  3. Barrett TJ, et al. 2021. J Thromb Haemost. 19:3139. PubMed
  4. Heger L, et al. 2018. Front Immunol. 9:744. PubMed
  5. Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
  6. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  7. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
RRID
AB_11219389 (BioLegend Cat. No. 354705)
AB_11219389 (BioLegend Cat. No. 354706)

Antigen Details

Structure
40 kD type II transmembrane glycoprotein, consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region
Distribution

Immature myeloid dendritic cells, activated macrophages

Function
Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, immune response
Interaction
Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Ligand/Receptor
Glycans containing terminal GalNAc moieties
Cell Type
Dendritic cells, Macrophages
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Van Vliet SJ, et al. 2006. Nat. Immunol. 7:1200.
2. Higashi N, et al. 2002. J. Biol. Chem. 277:20686.
3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.
4. Valladeau J, et al. 2001. J. Immunol. 167:5767.
5. Suzuki N, et al. 1996. J. Immunol. 156:128.

Gene ID
10462 View all products for this Gene ID
UniProt
View information about CD301 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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