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APC anti-human IFN-γ

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
4S.B3
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
4SB3_APC_IFN-gamma_ASR_020323
Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with 4S.B3 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • 4SB3_APC_IFN-gamma_ASR_020323
    Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with 4S.B3 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
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986702 500 µL $370
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
25 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Intracellular Staining for Flow Cytometry
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
  3. Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
  4. Gray P, et al. 1987. Lymphokines. 13:151.
Go To Top Version: 2    Revision Date: 03/06/2023

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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