- Clone
- TX83 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
141807 | 25 µg | $127 |
Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Transfected cells expressing allergin-1-Flag
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)
- RRID
-
AB_2561403 (BioLegend Cat. No. 141807)
Antigen Details
- Structure
- Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain
- Distribution
-
Mast cells, dendritic cells, macrophages, neutrophils
- Function
- Inhibits IgE-mediated degranulation of mast cells
- Interaction
- PTPN6, PTPN11 and INPP5D
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Hitomi K, et al. 2010. Nat. Immunol. 11:601.
2. Pirozzi G, et al. 1995. J. Immunol. 155:5811. - Gene ID
- 380732 View all products for this Gene ID
- UniProt
- View information about Allergin-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Allergin-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse Allergin-1 | TX83 | FC |
Brilliant Violet 421™ anti-mouse Allergin-1 | TX83 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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-
PE anti-mouse Allergin-1
C57BL/6 mouse peritoneal cavity cells were stained with c-Ki... -
Brilliant Violet 421™ anti-mouse Allergin-1
C57BL/6 mouse peritoneal cavity cells were stained with anti...
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