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APC anti-mouse CD201 (EPCR) Antibody

Pricing & Availability
Clone
RCR-16 (See other available formats)
Regulatory Status
RUO
Other Names
Endothelial protein C receptor, protein C receptor, PROCR, activated protein C receptor, APC receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RCR-16_APC_052412
Mouse endothelial cell line, bEND.3, was stained with mouse CD201 (clone RCR-16) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • RCR-16_APC_052412
    Mouse endothelial cell line, bEND.3, was stained with mouse CD201 (clone RCR-16) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
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141505 25 µg $131
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141506 100 µg $350
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Description

Endothelial protein C receptor (EPCR), also known as CD201, is a 46 kD N-glycosylated type I transmembrane protein, primarily expressed on the endothelial cells of arteries and veins in heart and lung. It is also expressed at high levels on a subset of hematopoietic stem cells and a subset of dendritic cells. CD201 functions as a primary receptor for protein C activation, and results in inhibition of both intrinsic and extrinsic coagulation pathways. It also plays an important role in many pathophysiologic processes, such as inflammation responses to infection, trauma, hematopoiesis, and autoimmune response. Deletion of the CD201 gene in knock-out mice leads to embryonic lethality before embryonic day 10, indicating that CD201 expression is critical for embryo development. In humans, mutations of CD201 have been associated with venous thromboembolism and myocardial infarction as well as with late fetal loss during pregnancy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking of protein C binding1.

Application References

(PubMed link indicates BioLegend citation)
  1. Centelles MN, et al. 2010. Thromb Haemost. 103:1239. (Block)
Product Citations
  1. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  2. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  3. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  4. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  5. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  6. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
RRID
AB_2561361 (BioLegend Cat. No. 141505)
AB_2561361 (BioLegend Cat. No. 141506)

Antigen Details

Structure
Type I transmembrane glycoprotein, 46 kD
Distribution

Endothelial cells, subset of hematopoietic stem cells, subset of dendritic cells

Function
Inhibits blood coagulation, apoptosis, inflammation, endothelial activation
Interaction
Protease-activated receptor 1 (Par-1)
Ligand/Receptor
Protein C, activated protein C
Cell Type
Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kerschen E, et al. 2010. J. Clin. Invest. 120:3167.
2. Iwasaki H, et al. 2010. Blood 116:544.
3. Balazs AB, et al. 2006. Blood 107:2317.

Gene ID
19124 View all products for this Gene ID
UniProt
View information about CD201 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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