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APC anti-mouse CD5 Antibody

Pricing & Availability
Clone
53-7.3 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-7.3_APC_CD5_Antibody_FC_062014.jpg
C57BL/6 mouse splenocytes were stained with CD5 (clone 53-7.3) APC (filled histogram) or rat IgG2a APC isotype control (open histogram).
  • 53-7.3_APC_CD5_Antibody_FC_062014.jpg
    C57BL/6 mouse splenocytes were stained with CD5 (clone 53-7.3) APC (filled histogram) or rat IgG2a APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
100625 25 µg $94
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100626 100 µg $292
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Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
  3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations
  1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  2. Worth AN, et al. 2022. Cell Rep. 39:110899. PubMed
  3. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  4. Han J, et al. 2018. BMC Res Notes. 11:229. PubMed
  5. Jennings EK, et al. 2021. STAR Protocols. 2(1):100284. PubMed
  6. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  7. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  8. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  9. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  10. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  11. Kim S, et al. 2021. Elife. 10:. PubMed
  12. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  13. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  14. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  15. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  16. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  17. Werner A, et al. 2021. iScience. 24:103076. PubMed
  18. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  19. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  20. Goc J, et al. 2021. Cell. 184:5015. PubMed
  21. Liang Z, et al. 2022. iScience. 25:105233. PubMed
  22. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
RRID
AB_2563928 (BioLegend Cat. No. 100625)
AB_2563928 (BioLegend Cat. No. 100626)

Antigen Details

Structure
Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution

Thymocytes, T cells, B-1 cells

Function
Negative regulator of T-B cell interaction
Ligand/Receptor
CD72, gp35-37
Cell Type
B cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.

Gene ID
12507 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org
Go To Top Version: 1    Revision Date: 07/01/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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