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APC anti-rat CD45RA Antibody

Pricing & Availability
Clone
OX-33 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte common antigen, high molecular weight form
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-33_APC_072710
LOU rat splenocytes stained with OX-33 APC
  • OX-33_APC_072710
    LOU rat splenocytes stained with OX-33 APC
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202313 25 µg $95
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202314 100 µg $220
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Description

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Leukocyte common antigen purified from rat splenocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Smith T, et al. 2000. Nature Med. 6:62. (IHC)
Product Citations
  1. Subas Satish HP, et al. 2022. MAbs. 14:2106621. PubMed
  2. Besseling PJ, et al. 2023. APL Bioeng. 7:026107. PubMed
  3. Noto FK, et al. 2020. PLoS One. 15:e0240169. PubMed
  4. Zimmerman KA, et al. 2021. Am J Physiol Renal Physiol. 321:F162. PubMed
RRID
AB_10639733 (BioLegend Cat. No. 202313)
AB_10639733 (BioLegend Cat. No. 202314)

Antigen Details

Structure
Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution

Primarily on B lymphocytes

Function
B cell receptor signal transduction
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.

Gene ID
24699 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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