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APC anti-rat CD86 Antibody

Pricing & Availability
Clone
24F (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
24F_APC_CD86_Antibody_2_010322
LPS-stimulated (3-day) LOU rat splenocytes were stained with anti-rat CD86 (clone 24F) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
  • 24F_APC_CD86_Antibody_2_010322
    LPS-stimulated (3-day) LOU rat splenocytes were stained with anti-rat CD86 (clone 24F) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
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Cat # Size Price Quantity Check Availability Save
200315 25 µg $102
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200316 100 µg $218
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Description

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV-1-transformed Lewis-S1 rat T cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).

Application References

(PubMed link indicates BioLegend citation)
  1. Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)
  2. Bluestone JA. 1995. Immunity 2:555. (IP, Block)
  3. Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
  4. Donius LR, et al. 2014. Immunobiology. 219:440. PubMed
Product Citations
  1. Pinho AG, et al. 2023. Int J Mol Sci. 24:. PubMed
RRID
AB_2910355 (BioLegend Cat. No. 200315)
AB_2910355 (BioLegend Cat. No. 200316)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T and B cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
  2. Hathcock KS, et al. 1993. Science. 262:905-7.
  3. Freeman GJ, et al. 1993. Science 262:907-9.
  4. Carreno BM, et al. 2002. Ann Rev Immunol. 20:29-53.
Gene ID
56822 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 01/03/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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