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APC/Cyanine7 anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
581_APCCyanine7_CD34_Antibody_013019_updated.png
Human peripheral blood mononuclear cells were stained with CD45 FITC, CD14 PE and CD34 (clone 581) APC/Cyanine7 (left) or Mouse IgG1, κ APC/Cyanine7 isotype control (right). Data shown was gated on the live, CD14 negative cell population.
  • 581_APCCyanine7_CD34_Antibody_013019_updated.png
    Human peripheral blood mononuclear cells were stained with CD45 FITC, CD14 PE and CD34 (clone 581) APC/Cyanine7 (left) or Mouse IgG1, κ APC/Cyanine7 isotype control (right). Data shown was gated on the live, CD14 negative cell population.
Compare all formats See APC/Cyanine7 spectral data
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343513 25 tests $153
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343514 100 tests $358
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  2. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
  3. Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed
  4. Takayama N, et al. 2020. Cell Stem Cell. 28(3):488-501.e10. PubMed
  5. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  6. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  7. Shukla S, et al. 2021. Nat Chem Biol. 17:784. PubMed
  8. Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed
  9. Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
  10. Lal A, et al. 2021. Nat Commun. 1507:12. PubMed
  11. Bigley V, et al. 2011. J Exp Med. 208:227. PubMed
  12. Montefiori LE, et al. 2021. Cancer Discov. 11:2846. PubMed
  13. Zhou X, et al. 2017. J Vasc Surg. 65:1161. PubMed
  14. Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed
  15. Dorrell C, et al. 2016. Nat Commun. 7:11756. PubMed
  16. Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed
  17. Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
  18. Bigley V, et al. 2015. J Leukoc Biol. 97:627. PubMed
  19. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
  20. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  21. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  22. Berent-Maoz B, et al. 2015. PLoS One. 10:125414. PubMed
  23. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
RRID
AB_1877169 (BioLegend Cat. No. 343513)
AB_1877169 (BioLegend Cat. No. 343514)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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