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APC/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_APCCy7_CD4_Antibody_011719.png
Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • RPA-T4_APCCy7_CD4_Antibody_011719.png
    Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
Compare all formats See APC/Cyanine7 spectral data
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300517 25 tests $116
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300518 100 tests $292
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  2. Imai H, et al. 2023. iScience. 26:106822. PubMed
  3. Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
  4. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  5. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  6. Yoshitomi H, et al. 2018. Nat Commun. 2.9875. PubMed
  7. Wefers C, et al. 2019. Front Immunol. 9:3156. PubMed
  8. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  9. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  10. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  11. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  12. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  13. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  14. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  15. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  16. Lübke M, et al. 2020. J Exp Med. 217:00:00. PubMed
  17. D, et al. 2016. Tuberculosis. 95: 470-475. PubMed
  18. Masuda H, et al. 2014. J Am Heart Assoc. 3:743. PubMed
  19. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  20. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  21. Iannetta M, et al. 2016. PLoS One. 11: 0160277. PubMed
  22. Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed
  23. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  24. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  25. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  26. Bilich T, et al. 2021. Science Translational Medicine. 13(590):. PubMed
  27. Naidoo KK, et al. 2020. PLoS One. 15:e0242448. PubMed
  28. García-Espinoza JA, et al. 2022. Curr Issues Mol Biol. 44:764. PubMed
  29. Heitmann JS, et al. 2021. Nature. Online ahead of print. PubMed
  30. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  31. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  32. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  33. Luo Y, et al. 2021. Front Immunol. 12:761209. PubMed
  34. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  35. Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed
  36. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  37. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  38. Jiang C, et al. 2020. Nature. 261:585. PubMed
  39. Einkauf KB, et al. 2022. Cell. 185:266. PubMed
  40. Brudno JN, et al. 2020. Nat Med. 270:26. PubMed
  41. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
  42. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  43. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID
AB_314085 (BioLegend Cat. No. 300517)
AB_314085 (BioLegend Cat. No. 300518)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD4 RPA-T4 FC
Biotin anti-human CD4 RPA-T4 FC
FITC anti-human CD4 RPA-T4 FC,SB
PE anti-human CD4 RPA-T4 FC
PE/Cyanine5 anti-human CD4 RPA-T4 FC
PE/Cyanine7 anti-human CD4 RPA-T4 FC
Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4 RPA-T4 FC
Alexa Fluor® 488 anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 647 anti-human CD4 RPA-T4 FC,SB
Pacific Blue™ anti-human CD4 RPA-T4 FC
Brilliant Violet 421™ anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 700 anti-human CD4 RPA-T4 FC,SB
PerCP anti-human CD4 RPA-T4 FC
PerCP/Cyanine5.5 anti-human CD4 RPA-T4 FC
Brilliant Violet 570™ anti-human CD4 RPA-T4 FC
Brilliant Violet 650™ anti-human CD4 RPA-T4 FC
Purified anti-human CD4 (Maxpar® Ready) RPA-T4 FC,CyTOF®
Alexa Fluor® 594 anti-human CD4 RPA-T4 FC,ICC
Brilliant Violet 510™ anti-human CD4 RPA-T4 FC
PE/Dazzle™ 594 anti-human CD4 RPA-T4 FC
Brilliant Violet 785™ anti-human CD4 RPA-T4 FC
Brilliant Violet 605™ anti-human CD4 RPA-T4 FC
Brilliant Violet 711™ anti-human CD4 RPA-T4 FC
APC/Fire™ 750 anti-human CD4 RPA-T4 FC
CD4 Fluorophore Sampler Kit RPA-T4 FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC RPA-T4 FC
TotalSeq™-A0072 anti-human CD4 RPA-T4 PG
TotalSeq™-B0072 anti-human CD4 RPA-T4 PG
TotalSeq™-C0072 anti-human CD4 RPA-T4 PG
Ultra-LEAF™ Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4 RPA-T4 PG
Go To Top Version: 5    Revision Date: 01/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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