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APC/Cyanine7 anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Regulatory Status
RUO
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
93_APCCyanine7_CD1632_Antibody_022519
BALB/c splenocytes stained with CD45R/B220 FITC and CD16/32 (clone 93) APC/Cyanine7 (left) or Rat IgG2a, λ APC/Cyanine7 isotype control (right).
  • 93_APCCyanine7_CD1632_Antibody_022519
    BALB/c splenocytes stained with CD45R/B220 FITC and CD16/32 (clone 93) APC/Cyanine7 (left) or Rat IgG2a, λ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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101327 25 µg $112
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101328 100 µg $323
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Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).

Application References

(PubMed link indicates BioLegend citation)
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  3. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  4. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  5. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  6. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  7. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  8. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  9. Li J, et al. 2019. JCI Insight. 5. PubMed
  10. Morales Torres C, et al. 2020. Nat Commun. 11:1792. PubMed
  11. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  12. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
  13. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  14. Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
  15. Lee SS, et al. 2022. Commun Biol. 5:654. PubMed
  16. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  17. Cho K, et al. 2021. iScience. 24:103117. PubMed
  18. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  19. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  20. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  21. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  22. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_1967102 (BioLegend Cat. No. 101327)
AB_1967102 (BioLegend Cat. No. 101328)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org
Go To Top Version: 3    Revision Date: 02/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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