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APC/Cyanine7 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
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Product Citations
publications
A20_APCCy7_CD45.1_Antibody_FC_120712
SJL mouse splenocytes were stained with CD45.1 (clone A20) APC/Cyanine7 (filled histogram) or mouse IgG2a APC/Cyanine7 isotype control (open histogram).
  • A20_APCCy7_CD45.1_Antibody_FC_120712
    SJL mouse splenocytes were stained with CD45.1 (clone A20) APC/Cyanine7 (filled histogram) or mouse IgG2a APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
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110715 25 µg $110
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110716 100 µg $253
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  2. VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed
  3. Zhao M, et al. 2022. Nat Commun. 13:7600. PubMed
  4. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  5. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  6. North K, et al. 2022. Nat Biotechnol. 40:1103. PubMed
  7. Morris V, et al. 2022. Cell Rep. 39:110752. PubMed
  8. Pulous FE, et al. 2022. Nat Neurosci. 25:567. PubMed
  9. Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed
  10. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  11. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  12. Le Q, et al. 2021. Clin Cancer Res. Online ahead of prin. PubMed
  13. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  14. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  15. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  16. Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed
  17. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  18. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  19. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  20. Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
  21. Amor C, et al. 2020. Nature. 583:127. PubMed
  22. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  23. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  24. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  25. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  26. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  27. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  28. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  29. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  30. Li J, et al. 2018. Immunity. 49:178. PubMed
  31. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  32. Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed
  33. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  34. De Simone G, et al. 2021. Immunity. :. PubMed
  35. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  36. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  37. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  38. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  39. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  40. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  41. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  42. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  43. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  44. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  45. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  46. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  47. Wang X, et al. 2022. Elife. 11:. PubMed
  48. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  49. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  50. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313504 (BioLegend Cat. No. 110715)
AB_313504 (BioLegend Cat. No. 110716)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 2    Revision Date: 01/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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