APC/Fire™ 750 anti-mouse H-2K<SUP>d</SUP>/H-2D<SUP>d</SUP> Antibody, H-2Kd/H-2Dd, 34-1-2S

Pricing & Availability

Clone: 34-1-2S (See other available formats)
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
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Product Citations: publications

A.
34-1-2S_APC-FIRE-750_H2K-H-2D_His-FC_122923 — C57BL/6 mouse splenocytes were stained with anti-mouse H-2K^d/H-2D^d (clone 34-1-2S) APC/Fire™ 750 (filled histogram) or mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).

Compare all formats See APC/Fire™ 750 spectral data

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Size

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114719

25 µg

$106

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114720

100 µg

$270

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Description

The 34-1-2S antibody reacts with the H-2K^d/H-2D^d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K^d/H-2D^d haplotype. H-2K^d/H-2D^d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation², complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
RRID: AB_3097451 (BioLegend Cat. No. 114719)
AB_3097451 (BioLegend Cat. No. 114720)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^d/H-2D^d haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821.

Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2Kd/H-2Dd on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

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Other Formats

View All H-2Kd/H-2Dd Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Alexa Fluor® 647 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PerCP/Cyanine5.5 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE/Cyanine7 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC/Fire™ 750 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Spark Violet™ 423 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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