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APC/Fire™ 810 anti-human CD20 Antibody

Pricing & Availability
Clone
S18015E (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S18015E_APC-FIRE-810_CD20_Antibody_1_071923
Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human CD20 (clone S18015E) APC/Fire™ 810 (left) or stained with anti-human CD19 Brilliant Violet 421™ only (right).
  • S18015E_APC-FIRE-810_CD20_Antibody_1_071923
    Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human CD20 (clone S18015E) APC/Fire™ 810 (left) or stained with anti-human CD19 Brilliant Violet 421™ only (right).
Compare all formats See APC/Fire™ 810 spectral data
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375529 25 tests $194
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375530 100 tests $393
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Description

CD20 (also known as B1 and Bp35) is a four transmembrane spanning protein measuring 33-37 kD. Expressed on pre-B cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. Involved in B-cell activation and is associated with a variety of Src family kinases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD20 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
RRID
AB_3068222 (BioLegend Cat. No. 375529)
AB_3068222 (BioLegend Cat. No. 375530)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Interaction
The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn).
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Hultin LE, et al. 1993. Cytometry 14:196.
  2. Tedder T and Engel P. 1994. Immunol. Today 15:450.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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