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APC/Fire™ 810 anti-human CD274 (B7-H1,PD-L1) Antibody

Pricing & Availability
Clone
MIH3 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH3_APCFire-810_CD274-_Antibody_031022
Human PBMCs were activated for 3 days with PHA and then stained with anti-human CD274 (clone MIH3) APC/Fire™ 810 (filled histogram) or mouse IgG1, κ APC/Fire™ 810 isotype control (open histogram).
  • MIH3_APCFire-810_CD274-_Antibody_031022
    Human PBMCs were activated for 3 days with PHA and then stained with anti-human CD274 (clone MIH3) APC/Fire™ 810 (filled histogram) or mouse IgG1, κ APC/Fire™ 810 isotype control (open histogram).
Compare all formats See APC/Fire™ 810 spectral data
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374515 25 tests $176
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374516 100 tests $429
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Description

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and non-hematopoietic cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PD-L1-transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include blocking1, 2.

Clone MIH3 blocks the binding of PD-L1 with PD-1 , but did not block the binding of PD-L1 with B7-1 (CD80).

Application References

(PubMed link indicates BioLegend citation)
  1. Haile S.T, et al. 2014. Cancer Immunol Res. 2:1. (Block)
  2. Butte M.J, et al. 2008. Mol Immunol. 45:3567. (Block)
RRID
AB_2927810 (BioLegend Cat. No. 374515)
AB_2927810 (BioLegend Cat. No. 374516)

Antigen Details

Structure
Type 1 transmembrane protein, member of the B7 family, 40kD.
Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells.

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (CD279), B7-1 (CD80).
Cell Type
T cells, B cells, NK cells, Monocytes, Macrophages, Granulocytes, Dendritic cells
Biology Area
Immunology, Cancer Biomarkers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Khan AR, et al. 2015. Nat Commun. 6:5997.
2. Kiyasu J, et al. 2015. Blood. 126:2193.
3. Herold M, et al. 2015 J Immunol. 195:3584.
4. Buddhisa S, et al. 2015. J Immunol. 194:4413.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 1    Revision Date: 03/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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