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Biotin anti-Allophycocyanin (APC) Antibody

Pricing & Availability
Clone
APC003 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_APC003_Biotin_111407
C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC
  • A_APC003_Biotin_111407
    C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC
  • B_APC003_Biotin_111407
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Cat # Size Price Quantity Check Availability Save
408003 25 µg $54
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408004 100 µg $118
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Description

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cyanine5.5 and APC/Cyanine7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
allophycocyanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations comparable to those of the primary antibody of interest. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Safi F 2023. STAR Protocols. 4(1):101965. PubMed
  2. Illingworth J, et al. 2013. J Immunol. 190:1038. PubMed
  3. Marangoni F, et al. 2021. Cell. . PubMed
  4. Coppieters K, et al. 2012. J Exp Med. 209:51:00. PubMed
  5. Matsushita H, et al. 2015. Cancer Immunol Res. 3:26. PubMed
RRID
AB_345359 (BioLegend Cat. No. 408003)
AB_345359 (BioLegend Cat. No. 408004)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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